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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
04/03/1998 |
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Data da última atualização: |
16/06/2017 |
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Autoria: |
SZASZ, A.; MITYKO, J. L.; ANDRASFALVY, A.; FARI, M. |
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Título: |
Methodological and genetic aspects of in vitro plant regeneration and genetic transformation of the recalcitrant pepper (capsicum annuum L.). |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 447, p.365-366, 1997. |
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Idioma: |
Inglês |
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Notas: |
Edição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996. |
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Conteúdo: |
The transfer of the exccellent regeneration capacity found in inbred line No. 40017 - 13 was aaattempted in commercial, non regenerable pepper varieties by sexual crossing. The obtained hybrids were genetically stabilized by the creation of dihaploid lines. results obtained in these experiments pr oved it is a way to improve the very poor regeneration ability ofparticular pepper varieties. Genetic transformation experiments were carried out on cotyledon and rooted hypocotyl explants of the No 40017 R -13 inbred line using a disamed Agrobacterium strain. the key step in the Agrobacterium mediated genetic transformation of pepper using cotyledons as explants was the elongation of the shoots surving the first kanamycin selection. It was observed that cefotaxim used to eliminate the bacteria has a phytohormone effect detrimental to this regeneration step. Interactions between antibiotics (cefotaxim, amoxycillin) and phytohormones (BAP, TDZ) during the regeneration process in cotyledon explants were revealed. |
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Palavras-Chave: |
Regeneracao in vitro. |
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Thesagro: |
Capsicum Annuum; Genética; Pimentão; Planta; Reprodução Vegetal. |
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Thesaurus Nal: |
genetic transformation; in vitro regeneration; pepper. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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Marc: |
LEADER 01952naa a2200277 a 4500
001 1133514
005 2017-06-16
008 1997 bl uuuu u00u1 u #d
100 1 $aSZASZ, A.
245 $aMethodological and genetic aspects of in vitro plant regeneration and genetic transformation of the recalcitrant pepper (capsicum annuum L.).
260 $c1997
500 $aEdição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.
520 $aThe transfer of the exccellent regeneration capacity found in inbred line No. 40017 - 13 was aaattempted in commercial, non regenerable pepper varieties by sexual crossing. The obtained hybrids were genetically stabilized by the creation of dihaploid lines. results obtained in these experiments pr oved it is a way to improve the very poor regeneration ability ofparticular pepper varieties. Genetic transformation experiments were carried out on cotyledon and rooted hypocotyl explants of the No 40017 R -13 inbred line using a disamed Agrobacterium strain. the key step in the Agrobacterium mediated genetic transformation of pepper using cotyledons as explants was the elongation of the shoots surving the first kanamycin selection. It was observed that cefotaxim used to eliminate the bacteria has a phytohormone effect detrimental to this regeneration step. Interactions between antibiotics (cefotaxim, amoxycillin) and phytohormones (BAP, TDZ) during the regeneration process in cotyledon explants were revealed.
650 $agenetic transformation
650 $ain vitro regeneration
650 $apepper
650 $aCapsicum Annuum
650 $aGenética
650 $aPimentão
650 $aPlanta
650 $aReprodução Vegetal
653 $aRegeneracao in vitro
700 1 $aMITYKO, J. L.
700 1 $aANDRASFALVY, A.
700 1 $aFARI, M.
773 $tActa Horticulturae, Leuven$gn. 447, p.365-366, 1997.
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